Description
Principle of the Assay::The Human CD154 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD154 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD154 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD154 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD154 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD154 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm